May 1, 2012

Tufts Imaging Facility

Please contact me by email Alenka.Lovy_Wheeler@tufts.edu or phone phone (617-636-3795) if you have any questions.

As of May 1, 2012, the confocals are operational. The Leica will be in the inverted configuration for the latter part of May

DAPI users: for your convenience, please consider using alternate dyes as nuclear stains if you want to use the Leica microscope.

If you are using two dyes (e.g. Alexa 488 and Alexa 561), you can get ToPro 3 (633nm excitation) to use as a nuclear stain, and image all dyes simultaneously (rather than scanning your DAPI separately). If you have Alexa 594--you will have cross-talk between this and the 633nm ToPro dye.

DAPI can be used with our new Nikon confocal that has a 405nm diode laser.

In addition to signing up to use the instrument on the Webevent calendar, please log your actual usage time in the blue notebook behind the instrument if it differs from your original time, or email me to change it.

*NEW* Research Equipment drive is available!! Your data will be available for 1-2 months and accessible from other computers as well so you don't always have to write a cd/dvd. Please see me for instructions on how to access the research drive remotely.

The internet and USB drives should not be used!! (to prevent viruses)

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